May 11, 2010

Midwest Biopolymers & Biocomposites Workshop

 

Poster Abstracts

109: Improved Method of Zein Extraction from Corn Gluten Meal to Increase Yield and Quality

Tim J. Anderson and Buddhi P. Lamsal

Department of Food Science and Human Nutrition
Iowa State University, Ames, Iowa


Zein extraction was modified to increase the yield and quality for biodegradable plastics. The commercial method from corn gluten meal (CGM) uses 88% w/w isopropanol and 0.25% NaOH; this method isolates a-zein with low yields of 20-24% w/w. The improved method used solvents tuned to extract high amounts of a, β, and γ-zeins. Solvents were 70% w/w isopropanol, 55% w/w isopropanol, and 70% v/v ethanol. Solvent concentrations were increased to 88% w/w isopropanol or 95% v/v ethanol leaving a-zein to be collected for precipitation at -20 °C. Experimental yields using 70% w/w aqueous isopropanol and 70% v/v aqueous ethanol were ~35% based on CGM(db). The yield for 88% w/w isopropanol was ~23%.